research cell line source s hek 293t  (ATCC)

Journal: npj Imaging

Article Title: [ 64 Cu]Cu-DOTA-TYPE7: a targeted PET radiotracer for imaging EphA2+ tumors

doi: 10.1038/s44303-026-00168-5

Figure Lengend Snippet: A and HEK-293T (EphA2-) ( B ) cell lines. Total binding experimental groups are indicated with a solid fill, whereas blocking groups with a 50x excess of unlabeled DOTA-TYPE7 are indicated using a patterned fill. Plots of [ 64 Cu]Cu-DOTA-TYPE7 internalization in PANC-1 ( C ) and HEK-293T ( D ) cell lines over 1 h.

Article Snippet: The PANC-1 and HEK-293T cell lines and all cell media were purchased from the American Type Culture Collection (ATCC) organization (Manassas, VA).

Techniques: Binding Assay, Blocking Assay

Journal: npj Imaging

Article Title: [ 64 Cu]Cu-DOTA-TYPE7: a targeted PET radiotracer for imaging EphA2+ tumors

doi: 10.1038/s44303-026-00168-5

Figure Lengend Snippet: Mice were administered either [ 64 Cu]Cu-DOTA-TYPE7 ( A , B , E , F ) or [ 64 Cu]Cu-DOTA-TYPE7 co-injected with a 25-fold excess of unlabeled DOTA-TYPE7 (C, D). Panels A – D show PET images and SUVs for PANC-1 tumors under binding ( A , B ) and blocking ( C , D ) conditions. Panels E-F depict PET images ( E ) and SUVs ( F ) for HEK-293T negative-control tumors.

Article Snippet: The PANC-1 and HEK-293T cell lines and all cell media were purchased from the American Type Culture Collection (ATCC) organization (Manassas, VA).

Techniques: Injection, Binding Assay, Blocking Assay, Negative Control

Journal: Redox Biology

Article Title: USP20 governs tyrosine kinase inhibitors resistance through ferroptosis evasion by targeting GPX4 in cancers

doi: 10.1016/j.redox.2026.104086

Figure Lengend Snippet: USP20 elevates GPX4 protein abundance through post-translational regulation. ( A ) Western blot analysis of GPX4 and USP20 proteins in 769-P and A549 cells transfected with lentivirus carrying shUSP20 or negative control. ( B ) Western blot analysis of GPX4 and USP20 proteins in 786-O and HEK 293T cells transfected with GST-USP20 plasmids or vector. ( C-D ) Analysis of GPX4 and USP20 mRNA expression in shScr and shUSP20 769-P and A549 cells. ( E-H ) shScr and shUSP20 769-P (E-F) and A549 (G-H) cells were treated with 100 μg/mL CHX at indicated time points. The GPX4 protein abundance was quantified by the Image J and statistical charts are produced by GraphPad. ( I-J ) shScr and shUSP20 769-P and A549 cells were treated with 20 μM MG132 for 6 h or 20 μM CQ for 12 h, and then assessed GPX4 expression. ( K ) Western blot analysis of GPX4 proteins in HEK293 cells transfected with GST-USP20 WT or GST-USP20 C154S plasmids. ( L-M ) Western blot analysis of GPX4 proteins in USP20 knockdown HEK293 cells transfected with GST-Vector, GST-USP20 WT or GST-USP20 C154S plasmids. The GPX4 protein abundance was quantified by the Image J software and statistical charts are produced by GraphPad. ( N ) Western blot analysis of GPX4 proteins in 769-P, SW839, A549, H1299 cells treated with indicated concentration GSK2643943A for 24 h. ( O–R ) The 769-P and A549 cells were treated with 10 μM GSK2643943A for 24 h, then 100 μg/mL CHX at indicated time points. The GPX4 protein abundance was quantified by the Image J software and statistical charts are produced by GraphPad. Data in C and D are presented as mean ± s.d. of n = 3 biological replicates.

Article Snippet: Human RCC cell lines 769-P, 786-O and SW839, human NSCLC cell lines A549, H1299 and the HEK-293T cell line were obtained from the American Type Culture Collection and cultured in RPMI-1640 or DMEM medium (Thermo Fisher Scientific, Inc.) added with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific, Inc.),100 U/ml penicillin and 0.1 mg/ml streptomycin (Thermo Fisher Scientific, Inc.) at a temperature of 37 °C.

Techniques: Quantitative Proteomics, Western Blot, Transfection, Negative Control, Plasmid Preparation, Expressing, Produced, Knockdown, Software, Concentration Assay